Nigerian Postgraduate Medical Journal

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 29  |  Issue : 3  |  Page : 236--243

Profiles of sugar fermenting bacteria of the oral cavity among children with dental caries attending stomatology services at Ruhengeri referral hospital in Musanze District, Northern Rwanda


Callixte Yadufashije1, Diane Uwase1, Liliane Muhimpundu2, Cedrick Izere1, Joseph Mucumbitsi1, Emmanuel Munyeshyaka1, Gratien Twagirumukiza1, Lydia Nzisa Mwanzia3, Hiberte Migabo1, Albert Onyango Mala2 
1 Department of Biomedical Laboratory Sciences, INES Ruhengeri Institute of Applied Sciences, Ruhengeri, Rwanda
2 Department of Medical Microbiology and Medical Laboratory Sciences, School of Biomedical Sciences, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya
3 Department of Midwifery and Gender, Moi University, Eldoret, Kenya

Correspondence Address:
Callixte Yadufashije
Associate Professor, Department of Biomedical Laboratory Sciences, INES Ruhengeri-Institute of Applied Sciences, P.O.Box: 155 Ruhengeri
Rwanda

Background: Dental caries remains a public health threat of concern among children. About 2.3 billion people are affected by dental caries, of which 530 million are children globally. Objective: This study was carried out to identify sugar fermenting bacteria in the oral cavity and their antibiotic susceptibility pattern, assess the association with sugar fermenter bacteria and dental caries and evaluate dental caries outcomes among children. Materials and Methods: This was a cross-sectional study conducted between October 2021 and February 2022 at Ruhengeri Referral Hospital. About 136 oral swab samples were collected from children with and without dental caries at 1:1 ratio. The samples were put in Stuart sterile container and transported to INES-clinical microbiology laboratory for microbial identification. Logistic regression analysis of demographic characteristics was performed to study the relationship between demographic variables and dental caries. Chi-square test was performed for the association between variables. Results: About 67.6% were male, while children of age 7–9 years (64.7%) dominated the age groups. Lactobacilli spp (15.29%) and Streptococcus mutans (12.94%) were the most predominant microorganisms observed in the oral cavity among children with dental caries. The S. mutans (x2 = 27.03, P < 0.00001, 95% confidence interval [CI]=0.2901–0.5785), S. aureus (x2 = 34.59, P < 0.00001, 95% CI = 0.3541–0.6292), Enterobacter aerogenes (x2 = 13.5, P = 0.000239, 95% CI = 0.151–0.4622), Serratia marcescens (x2 = 11.64, P = 0.00645, 95% CI = 0.1275–0.4418) and Klebsiella pneumonia (x2 = 13.51, P = 0.000237, 95% CI = 0.1511–0.4623) were significantly associated with dental caries. Teeth loss (x2 = 51.04, P < 0.00001, 95% CI = 0.4757–0.7205), teeth pain (x2 = 5.05, P = 0.0246, 95% CI = 0.0249–0.33499), and infection (x2 = 4.73, P = 0.02964, 95% CI = 0.0186–0.3441) were dental outcomes associated with tooth decay. Ciprofloxacin, clindamycin, and amoxicillin were the most sensitive antibiotics, while vancomycin and chloramphenicol were the most resistant. Conclusion: Sugar consumption favours the growth of sugar fermenter bacteria that cause dental caries among children. Dental caries is associated with adverse oral health outcomes among children. Oral health education is recommended for children. Parents are advised to reduce the consumption of sugary food for their children for oral health safety.


How to cite this article:
Yadufashije C, Uwase D, Muhimpundu L, Izere C, Mucumbitsi J, Munyeshyaka E, Twagirumukiza G, Mwanzia LN, Migabo H, Mala AO. Profiles of sugar fermenting bacteria of the oral cavity among children with dental caries attending stomatology services at Ruhengeri referral hospital in Musanze District, Northern Rwanda.Niger Postgrad Med J 2022;29:236-243


How to cite this URL:
Yadufashije C, Uwase D, Muhimpundu L, Izere C, Mucumbitsi J, Munyeshyaka E, Twagirumukiza G, Mwanzia LN, Migabo H, Mala AO. Profiles of sugar fermenting bacteria of the oral cavity among children with dental caries attending stomatology services at Ruhengeri referral hospital in Musanze District, Northern Rwanda. Niger Postgrad Med J [serial online] 2022 [cited 2022 Sep 28 ];29:236-243
Available from: https://www.npmj.org/article.asp?issn=1117-1936;year=2022;volume=29;issue=3;spage=236;epage=243;aulast=Yadufashije;type=0